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KMID : 0379119980260030354
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Korean Journal of Mycology
1998 Volume.26 No. 3 p.354 ~ p.360
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Isolation and Characterization of a Chitin Synthase Gene Fragments from Pleurotus sajor - caju
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박수철/Park, Soo Chul
류진창/유영복/정미정/김범기/Ryu, Jin Chang/Yoo, Young Bok/Jeong, Mi Jeong/Kim, Bum Gi
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Abstract
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We isolated three amplified DNA fragments from P. sajor-caju by Polmerase chain reaction (PCR) using the chithin synthase specific primers. Since the sequence analysis of the these fragments showed significant homology to the other known chitin synthase gene, we regarded these cloned fragments as PsCHS1, PsCHS2, and PsCHS3 according to their size. The PsCHS3, which showed the highest sequence homology (83% identity in amino acid level with ChsI of Rhizopus oligosporus in conserved region), was selected to see expression pattern of the corresponding gene. The result of RT-PCR using internal primer of the PsCHS3 fragment revealed that PsCHS3 gene was only expressed in cap and mycelium but not in stipe. In order to see whether the PsCHS3 gene was to be induced by wounding, the comparison of the mRNA level of this gene between wounded and unwounded mature cap showed at least two times induction of this gene by wounding treatment.
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